LSRII #2

 

The LSRII (#2) is capable of detecting up to 14 parameters (Forward Scatter, Side Scatter, and eleven fluorescence detectors).  It has three lasers for excitation of fluorochromes: a) a Coherent Sapphire laser for 488-nm excitation; b) a JDS Uniphase HeNe laser for 633-nm excitation; and c) a Coherent VioFlame for 405-nm excitation. In its basic configuration, the instrument is set up to detect 6 colors excited by the 488-nm laser, 3 colors excited by the 633-nm laser, and 3 colors excited by the 405-nm laser.  This instrument uses BD Biosciences FacsDiva software for acquisition and analysis.

Basic Configuration

Detector	Laser	Wavelength	Fluorochrome
A	488nm	750-810nm	PE Cy7
B	488nm	663-677nm	PE Cy5/PerCP/7-AAD
C	488nm	600-620nm	PE Texas Red
D	488nm	562-588nm	PE/PI
E	488nm	515-545nm	FITC/GFP/Alexa Fluor 488
F	488nm	SSC	------
A	405nm	600-620nm	Qdot 605
B	405nm	564-606nm	Qdot 585
C	405nm	425-475nm	Pacific Blue/DAPI
A	640nm	755-810nm	APC Cy7/Alexa Fluor 750
B	640nm	690-735nm	Alexa Fluor 700
C	640nm	650-670nm	APC

 

Scheduling

At this time, scheduling for analysis appointments on the LSRII must be scheduled through Research Core Facility personnel.  It is recommended that you schedule your appointment one week in advance.  All appointments are made on a first-come/first-served basis.  Please understand that last minute scheduling cannot be guaranteed, and is generally unavailable. 

If a scheduled analysis appointment is not met and the RCF staff has not been notified at least 2 hours prior to the appointment time, a fee of $50 will be charged to the investigator.