HUANG, Shile, Ph.D.
Biochemistry Molecular Biology
Room 7-213
Phone: 318-675-7759

Email: shuan1@Lsuhsc.edu

Research Interests

1. mTOR signaling in cell motility
2. Anticancer mechansim of curcumin
3. Cadmiun-induced neuronal apoptosis

Specialized Techniques/Procedures

1. cDNA cloning and site-directed mutagenesis
2. Construction of recombinant adenoviral/retroviral vectors
3. Analysis of mRNA expression using cDNA microarray, RT-PCR and Northern blotting
4. Knock-down of protein expression using RNA interference and antisense technology
5. Detection of proteins using SDS-PAGE, immunoprecipitation and immunoblotting
6. Enzyme, kinase and phosphatase assays
7. Cell culture including transfection/infection of mammalian cells
8. Analysis of cell proliferation, differentiation, migration and apoptosis
9. Visualization of protein distribution using immunohistochemistry and confocal laser  microscopy
10. In vivo animal experiments (tumor xenografts)