Louisiana State University Health Sciences Center - Shreveport
 Office Of Research
Vanchiere, John A., M.D., Ph.D.
5-302 /Medical School
Phone: 318-675-6081
Fax: 318-675-6059
Email: jvanch@lsuhsc.edu
Dr. Vanchiere received his M.D. and Ph.D. degree from Emory University(Atlanta, GA). He is a board certified Pediatric Infectious Diseases specialist who is trained in molecular virology, including completion of Ph.D. studies of neurotropic measles viruses at the Centers for Disease Control and postdoctoral studies of human polyomaviruses at Baylor College of Medicine. His expertise in translational research and clinical trial administration are particular assets for the conduct of this R24 project at the Squirrel Monkey Breeding and Research Resource. Dr. Vanchiere is currently an Associate Professor in the Department of Pediatrics at LSU Health Sciences Center in Shreveport. 1. Vanchiere, JA, Copeland, CM, Williams JT, Ruiz J, and Abee, CR (2010) Experimental Infection of Bolivian Squirrel Monkeys (Saimiri boliviensis boliviensis) with Human BK Polyomavirus. For submission to Journal of Infectious Diseases. 2. Vanchiere, JA, Copeland, CM, Lee CW, Williams, J.T, Ruiz, J, Williams, L, Abee, CR. (2010) Detection of Squirrel Monkey Polyomavirus in Feces of Captive-bred Squirrel Monkeys (Saimiri boliviensis). For submission to Journal of General Virology. 3. Vanchiere, JA and Casillas, AM. (2010) Urinary Cytokines in BK Viruria: Letter to the Editor. Transplantation, 89(9), in press. 4. Patel, P, Vanchiere, JA, Conrad, SA, Scott, LK. (2010) Use of therapeutic plasma exchange (TPE) as a rescue therapy in 2009 N1N1 influenza A-associated respiratory failure and hemodynamic shock. Pediatric Critical Care Medicine, in press.

Research Interests
1. The major goal of my laboratory is to understand the immunologic control of human polyomaviruses in pregnancy so that better diagnostic and treatment strategies can be developed for use in patient populations at risk for disease due to human polyomaviruses. 2. The second thrust of my laboratory is the development of a non-human primate model of human polyomavirus infection and disease for use in the evaluation of new therapeutic strategies for treatment of polyomavirus disease.

Specialized Techniques/Procedures
1. Qualitative PCR 2. Quantitative PCR 3. DNA cloning and sequencing 4. Multiplex cytokine analysis (Luminex) 5. gel electrophoresis 6. ELISA 7. viral culture 8. DNA extraction

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