Real-time
PCR
Two real time PCR instruments are available
in the Core Facility for PCR quantification of nucleic acids: an ABI Prism
7700 Sequence Detection System and a BioRad iCycler.
Laser Capture
Microdissection (LCM)
Laser Capture Microdissection (LCM) provides
a microdissection technology to locate a single cell or large groups of cells
and, using a simple aim-and-shoot method, extract them for subsequent molecular
analysis. It can be applied to any disease process, which is accessible through
tissue sampling, such as premalignant cancer lesions, multiple sclerosis,
arteriosclerosis, and Alzheimer's disease. Research applications include:
genomics (differential gene profiling, loss of heterozygosity, microsatellite
instability, and gene quantification) and proteomics (two-dimensional protein
gels , western blotting , and immuno-quantification of proteins).
DNA Array Analysis
The Core Facility has an Affymetrix GeneChip
Instrument and Bioinformatics Software System. The instrument system includes
a GeneArray Scannerä with workstation (Pentium III 500 MHz processor,
384 MB RAM, 25 GB hard drive, and 21? high resolution monitor), a model 400
GeneChip Fluidics Station, and a GeneChip Hybridization Oven 640. The Bioinformatics
System 1 consists of an Intell Xeon 4 x 450 MHz processor with 2 GB RAM and
110 GB hard drive array LIMS server, a second workstation, the GeneChip Analysis
Suite, the GeneChip Data Mining Tool, and a LIMS Access License.
Core Facilities
Histology
and Tissue Resource
Vector
Morphology
Fluorescence
Microscopy
Confocal Microscopy
Flow Cytometry
Mass Spectrometry/Proteomics
Automated Cellular Imaging System
Genomics
Real-time
PCR
Laser Capture Microdissection (LCM)
DNA Array Analysis
Animal Imaging
MicroPET
Imaging System
MicroCT Imaging System
Bioluminescence/
Fluorescence Imaging