Louisiana State University Health Sciences Center - Shreveport, Louisiana State University Health Sciences Center - Shreveport, LA
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Core Facility

Real-time PCR
     Two real time PCR instruments are available in the Core Facility for PCR quantification of nucleic acids: an ABI Prism 7700 Sequence Detection System and a BioRad iCycler.

Laser Capture Microdissection (LCM)
     Laser Capture Microdissection (LCM) provides a microdissection technology to locate a single cell or large groups of cells and, using a simple aim-and-shoot method, extract them for subsequent molecular analysis. It can be applied to any disease process, which is accessible through tissue sampling, such as premalignant cancer lesions, multiple sclerosis, arteriosclerosis, and Alzheimer's disease. Research applications include: genomics (differential gene profiling, loss of heterozygosity, microsatellite instability, and gene quantification) and proteomics (two-dimensional protein gels , western blotting , and immuno-quantification of proteins).

DNA Array Analysis

     The Core Facility has an Affymetrix GeneChip Instrument and Bioinformatics Software System. The instrument system includes a GeneArray Scannerä with workstation (Pentium III 500 MHz processor, 384 MB RAM, 25 GB hard drive, and 21? high resolution monitor), a model 400 GeneChip Fluidics Station, and a GeneChip Hybridization Oven 640. The Bioinformatics System 1 consists of an Intell Xeon 4 x 450 MHz processor with 2 GB RAM and 110 GB hard drive array LIMS server, a second workstation, the GeneChip Analysis Suite, the GeneChip Data Mining Tool, and a LIMS Access License.

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Core Facilities

Histology and Tissue Resource


Fluorescence Microscopy
Confocal Microscopy
Flow Cytometry
Mass Spectrometry/Proteomics
Automated Cellular Imaging System

Real-time PCR
Laser Capture Microdissection (LCM)
DNA Array Analysis

Animal Imaging
MicroPET Imaging System
MicroCT Imaging System
Fluorescence Imaging